The pepper genome editing module includes three function "SpacerSelect","OfftargetCheck" and "Guide RNA design".
The SpacerSelect tool identify spacers starting with "A" or "G" nucleotide on a given fasta sequences. It also identify off-target sites for each spacer. Due to the larget genome of pepper, this tool runs very slow.
The offtargetCheck tool idfentify offtargets for input spacer sequences. Multiple spacers in fasta format can be at the same time.
The GuideRNA design tool helps design primers for synthesizing guide RNA expression construct driven by U6 promoter.