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GENE EXPRESSION PROFILING IN DIFFERENT TISSUE AND DIFFERENT DEVELOPMENTAL STAGE. Hot pepper seeds were surface sterilized and then sown in 200-hole seedling tray filled with vermiculite. These trays were placed over a 30-liter lightproof plastic box filled with Japanese garden test nutrient solution, pH 6.0 (Hori, 1966). The seedlings were initially grown in growth room and were transplanted to 35 cm pots containing 10 kg moist soil in a glass house since one-month-old. Different pepper organs at different developmental stage were sampled and gene expression in each sample was profiled by high-through-put sequencing using Illumina Highseq4000 platform.